A single-step method for rapid extraction of total lipids from green microalgae.

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  Microalgae produce a wide range of lipid compounds of potential commercial interest. Total lipid extraction performed by conventional extraction methods, relying on the chloroform-methanol solvent system are too laborious and time consuming for screening large numbers of samples. In this study, three previous extraction methods devised by Folch et al. (1957), Bligh and Dyer […]

Functional and Antioxidant Properties of Plastic Bottle Caps Incorporated with BHA or BHT

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In this study, we prepared new antioxidant active plastic bottle caps by incorporating butylated hydroxyanisole (BHA) or butylated hydroxytoluene (BHT) and 2% (w/w) white masterbatch in high-density polyethylene (HDPE). Fourier-transform infrared (FT-IR) spectrometry revealed that the antioxidants and HDPE were uniformly mixed with noncovalent bonding. In addition, the differential scanning calorimetry (DSC) test revealed that […]

Direct Detection of Feline Coronavirus by Three Rapid Antigen Immunochromatographic Tests and by Real-Time PCR in Cat Shelters

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The aim of this study was the direct detection of feline coronavirus by real-time PCR and by three different rapid immunochromatographic (RIM) tests detecting antigens in faecal samples of shelter cats. Based on sensitivity and specificity calculated for each of the RIM tests, the utility of RIM tests was compared. Seventy faecal samples originating from […]

Diagnostic performance and characteristics of anterior nasal collection for the SARS-CoV-2 antigen test: a prospective study

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The clinical utility of antigen test using anterior nasal samples has not been well evaluated. We conducted a prospective study in a drive-through testing site located at a PCR center to evaluate the diagnostic performance of the antigen test QuickNavi-COVID19 Ag using anterior nasal samples and to compare the degrees of coughs or sneezes induction […]

Bean Extract-Based Gargle for Efficient Diagnosis of Active COVID-19 Infection Using Rapid Antigen Tests

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The antigen-based rapid diagnostic test (Ag-RDT) using saliva specimens is fast, noninvasive, and suitable for SARS-CoV-2 self-testing, unlike nasopharyngeal swab (NPS) testing. We evaluated a novel Beanguard gargle (BG)-based virus collection method that can be applied to Ag-RDT as an alternative to the current RT-PCR with an NPS for early diagnosis of COVID-19. This clinical […]

 Low-cost RNA extraction technique for extremely scalable transcriptome

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1bp1

Identification of diagnostic and prognostic biomarkers, and candidate focused brokers for hepatitis B virus-associated early stage hepatocellular carcinoma based mostly on RNA-sequencing information Major liver most cancers is a quickly progressing neoplasm with excessive morbidity and mortality charges. The current research aimed to determine potential diagnostic and prognostic biomarkers, and candidate focused brokers for hepatitis B […]

Metagenomic Insights into the Sewage RNA

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1bp1

The Interaction between Lengthy Noncoding RNAs and Proteins of the Epigenetic Equipment in Ovarian Most cancers Complete large-scale sequencing and bioinformatics analyses have uncovered a myriad of cancer-associated lengthy noncoding RNAs (lncRNAs). Aberrant expression of lncRNAs is related to epigenetic reprogramming throughout tumor growth and development, primarily attributable to their potential to work together with DNA, RNA, […]