A single-step method for rapid extraction of total lipids from green microalgae.
Microalgae produce a wide range of lipid compounds of potential commercial interest. Total lipid extraction performed by conventional extraction methods, relying on the chloroform-methanol solvent system are too laborious and time consuming for screening large numbers of samples. In this study, three previous extraction methods devised by Folch et al. (1957), Bligh and Dyer (1959) and Selstam and Öquist (1985) were compared and a faster single-step procedure was developed for extraction of total lipids from green microalgae. In the single-step procedure, 8 ml of a 2∶1 chloroform-methanol (v/v) mixture was added to fresh or frozen microalgal paste or pulverized dry algal biomass contained in a glass centrifuge tube.
The biomass was manually suspended by vigorously shaking the tube for a few seconds and 2 ml of a 0.73% NaCl water solution was added. Phase separation was facilitated by 2 min of centrifugation at 350 g and the lower phase was recovered for analysis. An uncharacterized microalgal polyculture and the green microalgae Scenedesmus dimorphus, Selenastrum minutum, and Chlorella protothecoides were subjected to the different extraction methods and various techniques of biomass homogenization. The less labour intensive single-step procedure presented here allowed simultaneous recovery of total lipid extracts from multiple samples of Centrifuge tube green microalgae with quantitative yields and fatty acid profiles comparable to those of the previous methods. While the single-step procedure is highly correlated in lipid extractability (r² = 0.985) to the previous method of Folch et al. (1957), it allowed at least five times higher sample throughput.
Santowhite, a commercial antioxidant used in the manufacture of polypropylene, contaminates 12-ml polyallomer tubes to the extent of 0.2-0.3 mg/tube.
- It is distributed through the plastic and appears as a microscopic dust on the tubes’ surfaces. In the preparation of polysomes from rat liver by standard methods, approximately 15% of the antioxidant in previously water-washed tubes was removed by suspension of the polysome pellet with stirring in 1-2 ml of Tris buffer, pH 7.8.
- The polyallomer impurity has been shown to be identical with Santowhite, which is 4,4′-butylidene-bis(6-tert-butyl-m-cresol), by UV, mass, and NMR spectra.
- It is not uniformly removed from polyallomer tubes by common detergents but is removed by washing with acetone, to which the plastic is resistant.
- Colloidal silica–aluminum modified–PVP density gradient centrifugation: centrifuge tube wall cell adherence, aggregation, separation properties and comparison to BSA and Ficoll.
A method is described for the inexpensive and easy preparation of colloidal silica–aluminum modified–polyvinylpyrrolidone (CS-AM-PVP) density gradient centrifugation medium.
Using density gradient centrifugation, several cell separation and biochemical characteristics were studied: centrifuge tube wall cell adherence, mouse spleen cell density distribution, rebanding properties, mitogen response and cell aggregation. Cell adherence to the centrifuge tube wall using density gradients of CS-AM-PVP was compared with density gradients of bovine serum albumin and Ficoll.
Few cells adhered to the centrifuge tube wall when CS-AM-PVP was used as a gradient medium; whereas, significant cell adherence to the centrifuge tube cell wall occurred when bovine serum albumin or Ficoll was used as a gradient medium. The CS-AM-PVP gradient medium did not inhibit the response to mitogens of mouse spleen cells which had been separated into density subpopulations in a discontinuous CS-AM-PVP density gradient, caused a minimum amount of cell aggregation, and was found to be non-toxic.
Islet transplantation is a promising method for restoring normoglycemia and alleviating the long term complications of diabetes.
- Widespread application of islet transplantation is hindered by the limited supply of human islets and requires a large increase in the availability of suitable insulin secreting tissue as well as robust quality assessment methodologies that can ensure safety and in vivo efficacy.
- We explore the application of nuclear magnetic resonance (NMR) spectroscopy in two areas relevant to beta cell engineering and islet transplantation: (1) the effect of genetic alterations on glucose metabolism, and (2) quality assessment of islet preparations prior to transplantation.
- Results obtained utilizing a variety of NMR techniques demonstrate the following: (1) Transfection of Rat1 cells with the c-myc oncogene (which may be involved in cell proliferation and cell cycle regulation) and overexpression of Bcl-2 (which may protect cells from stresses such as hypoxia and exposure to cytokines) introduce a wide array of alterations in cellular biochemistry, including changes in anaerobic and oxidative glucose metabolism, as assessed by 13C and 31P NMR spectroscopy.
- Overnight incubation of islets and beta cells in the bottom of centrifuge tubes filled with medium at room temperature, as is sometimes done in islet transportation, exposes them to severe oxygen limitations that may cause cell damage. Such exposure, leading to reversible or irreversible damage, can be observed with NMR-detectable markers using conventional 13C and 31P NMR spectroscopy of extracts.
- In addition, markers of irreversible damage (as well as markers of hypoxia) can be detected and quantified without cell extraction using high-resolution magic angle spinning 1H NMR spectroscopy. Finally, acute ischemia in a bed of perfused beta cells leads to completely reversible changes that can be followed in real time with 31P NMR spectroscopy
Plasma membranes were isolated from rat pheochromocytoma cells (PC-12) grown in spinner culture.
The rapid and simple isolation procedure consisted of a differential and isopycnic centrifugation (in a linear sucrose gradient) with the aid of a high capacity fixed angle rotor equipped with siliconized centrifuge tubes. The isolated membranes were closed and osmotically active vesicles (about 0.3 micron in diameter) with a mean intravesicular water space of 1.84 microliters/mg protein. In the presence of an inward gradient of sodium chloride and an outward gradient of potassium, [3H]noradrenaline (50 nM) was taken up and accumulated 550-fold (at 31 degrees C).
15 ml & 50 ml Centrifuge Tubes |
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TCF001-00-15mICentrifugeTubes450mlCentrifugeTubes4 | Vazyme | 15 mI Centrifuge Tubes*450 ml Centrifuge Tubes*4 | 1.09 EUR |
Centrifuge Tubes, 50 ml ,PP,Sterile,Plug-Seal Cap, in Bag |
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LC8005-500 | GenDepot | 500/CS | 120 EUR |
Centrifuge Tubes, 15 ml ,PP,Sterile,Plug-Seal Cap, in Bag |
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LC8003-500 | GenDepot | 500/CS | 102 EUR |
Centrifuge Tubes, 50 ml , Black ,PP,Sterile |
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LC8008-025 | GenDepot | 25/Bag | 35 EUR |
Centrifuge Tubes, 50 ml , Black ,PP,Sterile |
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LC8008-500 | GenDepot | 500/CS | 360 EUR |
Centrifuge Tubes, 50 ml , Amber ,PP,Sterile |
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LC8050-500 | GenDepot | 500/CS | 330 EUR |
Centrifuge Tubes, 15 ml , Black ,PP,Sterile |
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LC8007-050 | GenDepot | 50/Bag | 48 EUR |
Centrifuge Tubes, 15 ml , Black ,PP,Sterile |
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LC8007-500 | GenDepot | 500/CS | 260 EUR |
Centrifuge Tubes, 15 ml , Amber ,PP,Sterile |
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LC8015-500 | GenDepot | 500/CS | 330 EUR |
Centrifuge Tubes, 50 ml ,PP,Sterile,Plug-Seal Cap,Self Standing, in Bag |
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LC8006-500 | GenDepot | 500/CS | 215 EUR |
Centrifuge Tubes 0.65 ml Blue 10 bags of 1000 |
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MU11060 | Westburg | each | 527.56 EUR |
Centrifuge Tubes 0.65 ml Pink 10 bags of 1000 |
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MU11120 | Westburg | each | 527.56 EUR |
Centrifuge Tubes 1.7 ml Blue 10 bags of 500 |
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MU11430 | Westburg | each | 277.95 EUR |
Centrifuge Tubes 1.7 ml Pink 10 bags of 500 |
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MU11490 | Westburg | each | 277.95 EUR |
Centrifuge Tubes 2.0 ml Blue 10 bags of 400 |
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MU11920 | Westburg | each | 295.39 EUR |
Centrifuge Tubes 2.0 ml Pink 10 bags of 400 |
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MU11980 | Westburg | each | 295.39 EUR |
Rack S for centrifuge tubes 18 x 15 ml / 10 x 50 ml (45) |
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TP99017 | Westburg | each | Ask for price |
Rack L for centrifuge tubes 30 x 15 ml / 20 x 50 ml (30) |
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TP99019 | Westburg | each | Ask for price |
Centrifuge Tubes 0.65 ml Green 10 bags of 1000 |
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MU11080 | Westburg | each | 527.56 EUR |
Centrifuge Tubes 1.7 ml Green 10 bags of 500 |
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MU11450 | Westburg | each | 277.95 EUR |
Centrifuge Tubes 2.0 ml Green 10 bags of 400 |
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MU11940 | Westburg | each | 295.39 EUR |
Centrifuge Tubes 0.65 ml Purple 10 bags of 1000 |
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MU11170 | Westburg | each | 519.93 EUR |
Centrifuge Tubes 0.65 ml Yellow 10 bags of 1000 |
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MU11190 | Westburg | each | 527.56 EUR |
Centrifuge Tubes 1.7 ml Yellow 10 bags of 500 |
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MU11560 | Westburg | each | 275.23 EUR |
Centrifuge Tubes 2.0 ml Purple 10 bags of 400 |
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MU12020 | Westburg | each | 295.39 EUR |
Centrifuge Tubes 2.0 ml Yellow 10 bags of 400 |
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MU12040 | Westburg | each | 295.39 EUR |
50 mI Centrifuge Tubes |
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TCF00150-500Case | Vazyme | 500 / Case | 113.36 EUR |
Centrifuge Tubes 0.65 ml Natural 10 bags of 1000 |
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MU11150 | Westburg | each | 527.56 EUR |
Centrifuge Tubes 0.65 ml Rainbow 10 bags of 1000 |
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MU11210 | Westburg | each | 555.9 EUR |
Centrifuge Tubes 1.7 ml Natural 10 bags of 500 |
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MU11510 | Westburg | each | 273.59 EUR |
Centrifuge Tubes 1.7 ml Rainbow 10 bags of 500 |
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MU11590 | Westburg | each | 286.67 EUR |
Centrifuge Tubes 2.0 ml Natural 10 bags of 400 |
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MU12000 | Westburg | each | 291.03 EUR |
Centrifuge Tubes 2.0 ml Rainbow 10 bags of 400 |
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MU12060 | Westburg | each | 311.74 EUR |
Centrifuge Tubes 0.65 ml Natural 20 bags of 1000 |
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MU17030 | Westburg | each | 519.93 EUR |
Centrifuge Tubes 1.7 ml Natural 10 bags of 1000 |
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MU17040 | Westburg | each | 373.87 EUR |
Centrifuge Tubes 2.0 ml Natural 10 bags of 1000 |
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MU17050 | Westburg | each | 408.75 EUR |
15 mI Centrifuge Tubes |
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TCF00115-500Case | Vazyme | 500 / Case | 85.02 EUR |
Centrifuge tubes, glas, with round bottom, 50 ml |
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CD04-R50G | Phoenix instrument | each | 8 EUR |
15 mL Centrifuge Tube, Tubes and Caps Packed Separately, Non-Sterile, 50/pk, 500 |
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601151 | NEST Biotechnology | 500 pcs/cs | 37.29 EUR |
Centrifuge tubes, plastic, with round bottom, 50 ml |
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CD04-R50P | Phoenix instrument | each | 6 EUR |
50ml Conical Centrifuge Tubes, Green Cap, 25/Bag |
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CT788-G | Bio Basic | 1PK, 25UNIT | 67.31 EUR |
50ml Conical Centrifuge Tubes, Yellow Cap, 25/Bag |
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CT788-Y | Bio Basic | 1PK, 25UNIT | 67.31 EUR |
Block, 15 x1.5ml centrifuge tubes |
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BSH100-15 | Benchmark Scientific | 1 PC | 112.58 EUR |
Block, 15 x1.5ml centrifuge tubes |
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BCM1405-B15 | Bio Basic | 1 pcs, 1 UNIT | 194.76 EUR |
Small centrifuge, 6 x 50 ml, 4000 rpm, incl. rotor for 6 x 50 ml or 6 x 15 ml Tubes |
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CD-0412-50 | Phoenix instrument | each | 995 EUR |
Rack filled with 20 centrifuge tubes 50 ml, conical (10) |
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TP91550 | Westburg | each | Ask for price |
Stand For Centrifuge Tubes For 1.5 ml, |
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PW102-2NO | EWC Diagnostics | 1 unit | 8.01 EUR |
Rack filled with 30 centrifuge tubes 15 ml, conical (10) |
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TP91515 | Westburg | each | Ask for price |
Centrifuge Tubes 0.65 ml, low binding 10x500 |
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MU11300 | Westburg | each | 434.37 EUR |
Centrifuge Tubes, 50 ml ,PP,Sterile,Plug-Seal Cap, in Rack |
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LC8004-500 | GenDepot | 500/CS | 140 EUR |
Centrifuge Tubes 2.0 ml Natural 12 x 800 |
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MU1220 | Westburg | each | 566.8 EUR |
Corning LSE Single Block 5 x 50 mL Centrifuge Tubes - EACH |
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480129 | Scientific Laboratory Supplies | EACH | 145.8 EUR |
Dolphin Centrifuge Tubes 2.0 ml Natural 5x1000 |
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MU16140 | Westburg | each | 285.04 EUR |
29 mm Test Tube Foam Rack (for 50 mL centrifuge tubes) - EACH |
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MIX1118 | Scientific Laboratory Supplies | EACH | 54 EUR |
Centrifuge Tubes, 15 ml ,PP,Sterile,Plug-Seal Cap, in Rack |
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LC8002-500 | GenDepot | 500/CS | 260 EUR |
16 mm Test Tube Foam Rack (for 15 mL centrifuge tubes) - EACH |
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MIX1098 | Scientific Laboratory Supplies | EACH | 54 EUR |
Centrifuge Tubes 0.65 ml Natural Low binding 2x500 |
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MU11330 | Westburg | each | 93.74 EUR |
Centrifuge Tubes 2.0 ml Natural, low binding 4x500 |
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MU12160 | Westburg | each | 207.65 EUR |
Centrifuge Tubes 2.0 ml Natural Low binding 2x500 |
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MU12180 | Westburg | each | 104.64 EUR |
Centrifuge Tubes 1.7 ml Natural, Low binding 5x500 |
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MU39640T | Westburg | each | 218 EUR |
15 mL Centrifuge Tube Rack, 1/pk, 50/cs |
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610001 | NEST Biotechnology | 50 pcs/cs | 58.96 EUR |
Centrifuge tube 50 ml, conical / triple bagged (50) |
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TP391050 | Westburg | each | Ask for price |
Centrifuge Tubes 1.7 ml Natural Lubricated 2 x 500 |
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MU11720 | Westburg | each | 93.74 EUR |
4-Ways Microtube and Centrifuge Tubes Racks, Red |
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R023-R | Bio Basic | 1 UNIT | 64.18 EUR |
Greiner centrifuge tubes - PK300 |
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T2193-300EA | Scientific Laboratory Supplies | PK300 | 232.07 EUR |
4-Ways Microtube and Centrifuge Tubes Racks, Blue |
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R023-B | Bio Basic | 1 UNIT | 64.18 EUR |
Greiner centrifuge tubes - PK1000 |
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T1943-1000EA | Scientific Laboratory Supplies | PK1000 | 411.75 EUR |
4-Ways Microtube and Centrifuge Tubes Racks, Yellow |
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R023-Y | Bio Basic | 1 UNIT | 64.18 EUR |
Centrifuge tube 50 ml, round (180) |
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TP91056 | Westburg | each | Ask for price |
4-Ways Microtube and Centrifuge Tubes Racks, Natural |
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R023-N | Bio Basic | 1 UNIT | 64.18 EUR |
Block, 15 x 1.5ml or 2.0ml centrifuge tubes (conical) |
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BSH100-1520 | Benchmark Scientific | 1 PC | 112.58 EUR |
Block, 15 x 1.5ml or 2.0ml centrifuge tubes (conical) |
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BCM1405-B1520 | Bio Basic | 1 pcs, 1 UNIT | 194.76 EUR |
Centrifuge Tubes, Autoclavable BLUE |
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CG264-1X100NO | EWC Diagnostics | 1 unit | 12.63 EUR |
Centrifuge tube 50 ml, conical (360) |
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TP91050 | Westburg | each | Ask for price |
Centrifuge tube 50 ml, conical (160) |
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TP91054 | Westburg | each | Ask for price |
Centrifuge Tubes, Autoclavable Round |
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CG276-1X100NO | EWC Diagnostics | 1 unit | 23.15 EUR |
Centrifuge Tubes, Autoclavable Round |
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CG277-1X100NO | EWC Diagnostics | 1 unit | 12.79 EUR |
Centrifuge Tubes, Autoclavable Round |
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CG278-1X100NO | EWC Diagnostics | 1 unit | 9.07 EUR |
Centrifuge Tubes, Autoclavable Round |
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CG279-1X100NO | EWC Diagnostics | 1 unit | 3.24 EUR |
Block for 5ml centrifuge tubes |
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H5000-5MT | Benchmark Scientific | 1 PC | 494.19 EUR |
15 mL Centrifuge Tube, Bulk, Sterile, 50/pk, 500/cs |
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601052 | NEST Biotechnology | 500 pcs/cs | 39.82 EUR |
Centrifuge tube 15 ml, conical (360) |
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TP91014 | Westburg | each | Ask for price |
Centrifuge tube 15 ml, conical (800) |
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TP91015 | Westburg | each | Ask for price |
Centrifuge tube 15 ml, conical (800) |
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TP91115 | Westburg | each | Ask for price |
Centrifuge Tubes, Autoclavable Conical |
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CG274-100X1NO | EWC Diagnostics | 1 unit | 32.38 EUR |
15mL Sterile Centrifuge Tubes PP |
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ARG1818 | Scientific Laboratory Supplies | PK500 | 247.5 EUR |
50mL Sterile Centrifuge Tubes PP |
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ARG1824 | Scientific Laboratory Supplies | PK500 | 339.57 EUR |
Block, 20x10mm test tubes or 20x2.0ml centrifuge tubes |
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BSW10 | Benchmark Scientific | 1 PC | 149.04 EUR |
Block, 20x10mm test tubes or 20x2.0ml centrifuge tubes |
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BCM1412-BSW10 | Bio Basic | 1 pcs, 1 UNIT | 233.21 EUR |
Eppendorf Adapter for 0.5 mL micro- centrifuge tubes + 0.6 ml Microtainer set of 6 - PK6 |
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E5425716001 | Scientific Laboratory Supplies | PK6 | 17.48 EUR |
Centrifuge tube 15 ml, conical / triple bagged (100) |
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TP391015 | Westburg | each | Ask for price |
CapLock 0.5mL Centrifuge Tubes - PK500 |
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ARG1782 | Scientific Laboratory Supplies | PK500 | 74.03 EUR |
Oakridge Centrifuge Tubes 30ml - PK10 |
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31390030 | Scientific Laboratory Supplies | PK10 | 307.8 EUR |
CapLock 1.5mL Centrifuge Tubes - PK1000 |
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ARG1808 | Scientific Laboratory Supplies | PK1000 | 132.19 EUR |
50 mL Centrifuge Tube Rack, 1/pk, 50/cs |
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610101 | NEST Biotechnology | 50 pcs/cs | 58.96 EUR |
Block, 24 x 0.5ml centrifuge tubes |
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BSH100-05 | Benchmark Scientific | 1 PC | 112.58 EUR |
Block, 24 x 0.5ml centrifuge tubes |
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BSW05 | Benchmark Scientific | 1 PC | 149.04 EUR |
Block, 24 x 0.5ml centrifuge tubes |
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BCM1405-B05 | Bio Basic | 1 pcs, 1 UNIT | 194.76 EUR |
Block, 24 x 0.5ml centrifuge tubes |
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BCM1412-BSW05 | Bio Basic | 1 pcs, 1 UNIT | 233.21 EUR |
Centrifuge Tubes S 2420 30ml Qfit - PK10 |
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QBC24C24R0 | Scientific Laboratory Supplies | PK10 | 270 EUR |
Centrifuge Tubes, Autoclavable RED Cap, |
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CG272-1X100NO | EWC Diagnostics | 1 unit | 31.16 EUR |
Block, 4 x 15ml centrifuge tubes |
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BSH100-150 | Benchmark Scientific | 1 PC | 142.76 EUR |
Block, 2 x 50ml centrifuge tubes |
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BSH100-500 | Benchmark Scientific | 1 PC | 142.76 EUR |
Block, 12 x 15ml centrifuge tubes |
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BSW15 | Benchmark Scientific | 1 PC | 149.04 EUR |
Block, 5 x 50ml centrifuge tubes |
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BSW50 | Benchmark Scientific | 1 PC | 149.04 EUR |
Block, 4 x 15ml centrifuge tubes |
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BCM1405-B150 | Bio Basic | 1 pcs, 1 UNIT | 227.29 EUR |
Block, 2 x 50ml centrifuge tubes |
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BCM1405-B500 | Bio Basic | 1 pcs, 1 UNIT | 227.29 EUR |
Block, 12 x 15ml centrifuge tubes |
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BCM1412-BSW15 | Bio Basic | 1 pcs, 1 UNIT | 233.21 EUR |
Block, 5 x 50ml centrifuge tubes |
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BCM1412-BSW50 | Bio Basic | 1 pcs, 1 UNIT | 233.21 EUR |
Centrifuge Tubes, with ring (leak proof) |
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PW1207-1X100NO | EWC Diagnostics | 1 unit | 21.38 EUR |
Centrifuge Tubes, Autoclavable With RED |
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CG271-1X100NO | EWC Diagnostics | 1 unit | 40.01 EUR |
Block for 5mL centrifuge tubes - EACH |
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MIX7218 | Scientific Laboratory Supplies | EACH | 714.15 EUR |
Block, 24 x1.5ml centrifuge tubes (conical) |
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BSW1500 | Benchmark Scientific | 1 PC | 149.04 EUR |
Block, 24 x1.5ml centrifuge tubes (conical) |
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BCM1412-BSW1500 | Bio Basic | 1 pcs, 1 UNIT | 233.21 EUR |
15mL Sterile Centrifuge Tubes PP - PK500 |
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ARG1814 | Scientific Laboratory Supplies | PK500 | 304.04 EUR |
50mL Sterile Centrifuge Tubes PP - PK500 |
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ARG1820 | Scientific Laboratory Supplies | PK500 | 411.26 EUR |
Falcon Conical 50ml PP Centrifuge Tubes Bagged - PK500 |
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352070 | Scientific Laboratory Supplies | PK500 | 226.8 EUR |
Falcon Conical 15ml PP Centrifuge Tubes Bagged - PK500 |
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352096 | Scientific Laboratory Supplies | PK500 | 193.05 EUR |
15ml Conical Centrifuge Tubes, Polypropylene |
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28-101 | Genesee Scientific | 20 Racks of 25 Tubes, 500/Unit | 89.45 EUR |
15ml Conical Centrifuge Tubes, Polypropylene |
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28-103 | Genesee Scientific | 20 Bags of 25 Tubes, 500/Unit | 74.7 EUR |
50ml Conical Centrifuge Tubes, Polypropylene |
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28-106 | Genesee Scientific | 20 Racks of 25 Tubes, 500/Unit | 107.43 EUR |
50ml Conical Centrifuge Tubes, Polypropylene |
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28-108 | Genesee Scientific | 20 Bags of 25 Tubes, 500/Unit | 85.75 EUR |
50ml Conical Centrifuge Tubes, Polypropylene |
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21-106 | Genesee Scientific | 20 Racks of 25 Tubes, 500/Unit | 101.53 EUR |
50ml Conical Centrifuge Tubes, Polypropylene |
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21-108 | Genesee Scientific | 20 Bags of 25 Tubes, 500/Unit | 81.01 EUR |
50ml Plug-Seal Centrifuge Tubes with Rack, 25 tubes/rack |
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10-0501 | IHC World | - | 12.5 EUR |
Kimas high strength centrifuge tubes - PK6 |
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Z514861-6EA | Scientific Laboratory Supplies | PK6 | 116.1 EUR |
5 mL Centrifuge Tubes, Clear, Round, Lock Cap, Sterile, 200/pk, 2000/cs |
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603111 | NEST Biotechnology | 2000 pcs/cs | 109.56 EUR |
Centrifuge 5804 + Rotor S-4-72 + adapters 15/50ml tubes - EACH |
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E5804000565 | Scientific Laboratory Supplies | EACH | 7171.2 EUR |
Tubes Centrifuge 15ml Conical - PK500 |
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CEN9202 | Scientific Laboratory Supplies | PK500 | 259.2 EUR |
CENTRIFUGE TUBE 50 ML 30 X 115 MM - PK500 |
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T2318-500EA | Scientific Laboratory Supplies | PK500 | 334.89 EUR |
15 mL Centrifuge Tube, Rack(PS Rack), Sterile, 50/pk, 500/cs |
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601051 | NEST Biotechnology | 500 pcs/cs | 43.01 EUR |
Centrifuge 5804R + Rotor S-4-72 + adapters 15/50ml tubes - EACH |
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E5805000665 | Scientific Laboratory Supplies | EACH | 10750.05 EUR |
Centrifuge 5810 + Rotor S-4-104 + adapters 15/50ml tubes - EACH |
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E5810000560 | Scientific Laboratory Supplies | EACH | 10126.35 EUR |
250 mL PP Centrifuge Tubes with Plug Seal Cap, Sterile, 6/pk, 102/cs |
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622001 | NEST Biotechnology | 102 pcs/cs | 129.8 EUR |
500 mL PP Centrifuge Tubes with Plug Seal Cap, Sterile, 6/pk, 36/cs |
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623001 | NEST Biotechnology | 36 pcs/cs | 61.05 EUR |
Centrifuge 5810R + Rotor S-4-104 + adapters 15/50ml tubes - EACH |
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E5811000660 | Scientific Laboratory Supplies | EACH | 13965.75 EUR |
SINGLE BLOCK, 12 X 15ML CENTRIFUGE TUBES |
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480124 | CORNING | 1/pk | 136.8 EUR |
Centrifuge tube 50 ml, conical, with rim (320) |
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TP91051 | Westburg | each | Ask for price |
Block, 24 x 1.5ml or 2.0ml centrifuge tubes |
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BSW1520 | Benchmark Scientific | 1 PC | 149.04 EUR |
Block, 24 x 1.5ml or 2.0ml centrifuge tubes |
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BCM1412-BSW1520 | Bio Basic | 1 pcs, 1 UNIT | 233.21 EUR |
250mL centrifuge tube PP (59.8 X 160mm), with screw-cap, 14 bags of 5 tubes |
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C1250 | MTC Bio | 70 | 234 EUR |
Block, 15 x 1.5ml Centrifuge Tubes for Dry Bath with Heated Lid |
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BSH100-15HL | Benchmark Scientific | 1 PC | 112.58 EUR |
Block, 15 x 1.5ml Centrifuge Tubes for Dry Bath with Heated Lid |
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BCM1405-B15HL | Bio Basic | 1 pcs, 1 UNIT | 194.76 EUR |
Falcon Conical 225ml PP Centrifuge Tubes - PK48 |
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352075 | Scientific Laboratory Supplies | PK48 | 149.85 EUR |
5 mL Centrifuge Tubes, Clear, Round, Lock Cap, Non-Sterile, 200/pk, 2000/cs |
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603011 | NEST Biotechnology | 2000 pcs/cs | 99.99 EUR |
15mL and 50ml Universal Rack for Conical Bottom Centrifuge Tubes - PK20 |
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4366 | Scientific Laboratory Supplies | PK20 | 58.05 EUR |
Falcon Conical 15ml Ps Centrifuge Tubes - PK500 |
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352099 | Scientific Laboratory Supplies | PK500 | 221.4 EUR |
5-Well Centrifuge Tube Box (1.5ml To 2ml Tubes) |
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R005-G | Bio Basic | 1 UNIT | 61.57 EUR |
High Speed Centrifuge, Adapt for 1.5/2.0 mL Microcentrifuge Tubes, 500-1200 RPM |
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102101 | NEST Biotechnology | 1 pcs/cs | 184.36 EUR |
250 mL PP Centrifuge Tubes with Plug Seal Cap, Racked, Sterile, 6/pk, 24/cs |
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622002 | NEST Biotechnology | 24 pcs/cs | 38.17 EUR |
500 mL PP Centrifuge Tubes with Plug Seal Cap, Racked, Sterile, 6/pk, 24/cs |
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623002 | NEST Biotechnology | 24 pcs/cs | 45.65 EUR |
Block, 6 x 5ml centrifuge tubes (17mm diameter) |
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BSH100-5MT | Benchmark Scientific | 1 PC | 142.76 EUR |
Block, 12 x 5.0ml centrifuge tubes (17mm diameter) |
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BSW5MT | Benchmark Scientific | 1 PC | 149.04 EUR |
Block, 6 x 5ml centrifuge tubes (17mm diameter) |
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BCM1405-B5MT | Bio Basic | 1 pcs, 1 UNIT | 227.29 EUR |
Block, 12 x 5.0ml centrifuge tubes (17mm diameter) |
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BCM1412-BSW5MT | Bio Basic | 1 pcs, 1 UNIT | 233.21 EUR |
Block, 8x5ml centrifuge tubes, for MultiTherm Touch |
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H5100-5MT | Benchmark Scientific | 1 each | 374.92 EUR |
Block 15 x 1.5mL Centrifuge Tubes for Dry Bath with Heated Lid - EACH |
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BLO1254 | Scientific Laboratory Supplies | EACH | 238.95 EUR |
Centrifuge Tube, 5ml, 200 per. pk. (4 bags of 50) |
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C1005-T5-Clear | Benchmark Scientific | 1 each | 44.5 EUR |
Centrifuge Tube, 5ml, 200 per. pk. (4 bags of 50) |
|||
C1005-T5_Clear | Benchmark Scientific | each | 44.5 EUR |
Centrifuge 5804 + Rotor S-4-72 + adapters 15/50ml tubes- IVD Only - EACH |
|||
E5804000560 | Scientific Laboratory Supplies | EACH | 8100 EUR |
Centrifuge 5804R + Rotor S-4-72 + adapters 15/50ml tubes- IVD Only - EACH |
|||
E5805000661 | Scientific Laboratory Supplies | EACH | 11284.65 EUR |
Centrifuge 5810 + Rotor S-4-104 + adapters 15/50ml tubes- IVD Only - EACH |
|||
E5810000564 | Scientific Laboratory Supplies | EACH | 10631.25 EUR |
15ml Centrifuge Tube, Screw Cap, Natural, 100/Bag |
|||
BT888-N | Bio Basic | 1PK, 100UNIT | 74.29 EUR |
Centrifuge 5810R + Rotor S-4-104 + adapters 15/50ml tubes- IVD Only - EACH |
|||
E5811000665 | Scientific Laboratory Supplies | EACH | 13902.3 EUR |
50 mL Centrifuge Tube, Bulk, Sterile, 25/pk, 500/cs |
|||
602052 | NEST Biotechnology | 500 pcs/cs | 63.91 EUR |
50mL Rack for Conical Bottom Centrifuge Tubes - PK20 |
|||
4365 | Scientific Laboratory Supplies | PK20 | 58.05 EUR |
15mL Rack for Conical Bottom Centrifuge Tubes - PK20 |
|||
431355 | Scientific Laboratory Supplies | PK20 | 45.9 EUR |
Falcon Conical 175ml PP Centrifuge Tubes Racked - PK48 |
|||
352076 | Scientific Laboratory Supplies | PK48 | 141.75 EUR |
Cool rack 9 wells for 15ml centrifuge tubes - EACH |
|||
HOM2740 | Scientific Laboratory Supplies | EACH | 1120.69 EUR |
Cool rack 4 wells for 50ml centrifuge tubes - EACH |
|||
HOM2742 | Scientific Laboratory Supplies | EACH | 1120.69 EUR |
Falcon Conical 15ml PP Centrifuge Tubes Racked - PK500 |
|||
352097 | Scientific Laboratory Supplies | PK500 | 245.7 EUR |
Falcon Conical 50ml PP Centrifuge Tubes Racked - PK500 |
|||
352098 | Scientific Laboratory Supplies | PK500 | 260.55 EUR |
Kimax Reuseable Glass Centrifuge tubes oil 203mm - PK12 |
|||
TUB2716 | Scientific Laboratory Supplies | PK12 | 977.4 EUR |
15 mL Centrifuge Tube, Amber Color, Rack(PS Rack), Sterile, 50/pk, 500/cs |
|||
601201 | NEST Biotechnology | 500 pcs/cs | 67.54 EUR |
50ml Skirted Centrifuge Tube, Green Cap, 25/Bag |
|||
TK943-G | Bio Basic | 1PK, 25UNIT | 67.31 EUR |
Block 6 x 5mL centrifuge tubes (17mm diameter) - EACH |
|||
BLO1256 | Scientific Laboratory Supplies | EACH | 207.9 EUR |
Block 12 x 5.0mL centrifuge tubes (17mm diameter) - EACH |
|||
BLO1276 | Scientific Laboratory Supplies | EACH | 218.7 EUR |
50ml Skirted Centrifuge Tube, Yellow Cap, 25/Bag |
|||
TK943-Y | Bio Basic | 1PK, 25UNIT | 67.31 EUR |
Falcon Conical 15ml Ps Multipack Centrifuge Tubes - PK500 |
|||
352095 | Scientific Laboratory Supplies | PK500 | 226.8 EUR |
Centrifuge tube 13 ml, flat (800) |
|||
TP91019 | Westburg | each | Ask for price |
15ml Centrifuge Tube, Screw Cap, Red, Sterile, 100/Bag |
|||
BT888-SR | Bio Basic | 1PK, 100UNIT | 82.27 EUR |
15ml Centrifuge Tube, Screw Cap, Blue, Sterile, 100/Bag |
|||
BT888-SB | Bio Basic | 1PK, 100UNIT | 87.42 EUR |
CoolRack XT 5ml holds 12 x 5ml centrifuge tubes - EACH |
|||
432060 | Scientific Laboratory Supplies | EACH | 394.2 EUR |
50 ml Centrifuge Tube with Flat Cap, Bulk, Sterile (20x25) |
|||
OLOCT-50B | Westburg | each | 180.94 EUR |
15mL Flip Top Centrifuge Tubes Sterile DNase Free - PK500 |
|||
ARG1154 | Scientific Laboratory Supplies | PK500 | 405 EUR |
50mL Flip Top Centrifuge Tubes Sterile DNase Free - PK500 |
|||
ARG1156 | Scientific Laboratory Supplies | PK500 | 537.3 EUR |
Benchmark Centrifuge Tube Red 5mL (4 bags of 50) - PK200 |
|||
CEN2048 | Scientific Laboratory Supplies | PK200 | 67.5 EUR |
15ml Centrifuge Tube, Screw Cap, Yellow, Sterile, 100/Bag |
|||
BT888-SY | Bio Basic | 1PK, 100UNIT | 82.27 EUR |
Snap Cap Polyethylene For Centrifuge Tubes Size 2 - PK500 |
|||
TUB2728 | Scientific Laboratory Supplies | PK500 | 97.2 EUR |
15 ml Centrifuge Tube with Flat Cap, Bulk, Sterile (20x25) |
|||
OLOCT-15B | Westburg | each | 148.24 EUR |
50 ml Centrifuge Tube with Flat Cap, Racked, Sterile (20x25) |
|||
OLOCT-50R | Westburg | each | 196.2 EUR |
15ml Centrifuge Tube, Screw Cap, Natural, Sterile, 100/Bag |
|||
BT888-SN | Bio Basic | 1PK, 100UNIT | 82.94 EUR |
Benchmark Centrifuge Tube Blue 5mL (4 bags of 50) - PK200 |
|||
CEN2046 | Scientific Laboratory Supplies | PK200 | 67.5 EUR |
Benchmark Centrifuge Tube Clear 5mL (4 bags of 50) - PK200 |
|||
CEN2045 | Scientific Laboratory Supplies | PK200 | 67.5 EUR |
Benchmark Centrifuge Tube Green 5mL (4 bags of 50) - PK200 |
|||
CEN2047 | Scientific Laboratory Supplies | PK200 | 67.5 EUR |
Benchmark Centrifuge Tube Black 5mL (4 bags of 50) - PK200 |
|||
CEN2051 | Scientific Laboratory Supplies | PK200 | 67.37 EUR |
15 ml Centrifuge Tube with Flat Cap, Racked, Sterile (20x25) |
|||
OLOCT-15R | Westburg | each | 174.4 EUR |
50ml High Speed Centrifuge Tube, Natural cap, 25/Bag |
|||
CT790-N | Bio Basic | 1PK, 25UNIT | 72.64 EUR |
Benchmark Centrifuge Tube Yellow 5mL (4 bags of 50) - PK200 |
|||
CEN2049 | Scientific Laboratory Supplies | PK200 | 67.5 EUR |
50 mL Centrifuge Tube, Rack (PS Rack), Sterile, 25/pk, 500/cs |
|||
602051 | NEST Biotechnology | 500 pcs/cs | 74.25 EUR |
50ml Skirted Centrifuge Tube, Green Cap, Sterile, 25/Bag |
|||
TK943-GS | Bio Basic | 1PK, 25UNIT | 69.72 EUR |
Centrifuge tube 16 ml, round, long (800) |
|||
TP91017 | Westburg | each | Ask for price |
50ml Skirted Centrifuge Tube, Yellow Cap, Sterile, 25/Bag |
|||
TK943-YS | Bio Basic | 1PK, 25UNIT | 69.72 EUR |
Centrifuge tube 13 ml, round, short (800) |
|||
TP91016 | Westburg | each | Ask for price |
Corning 15mL PP Centrifuge Tubes Rack Packed with CentriStar Cap Sterile 50/Rack 500/Case - PK500 |
|||
430790 | Scientific Laboratory Supplies | PK500 | 248.4 EUR |
15ml centrifuge tubes, rack pack, sterile, flat top caps, graduation |
|||
SGB011-R | SEEN GROUP LIMITED | 50pcs/rack, 500pcs/carton | 40 EUR |
50ml centrifuge tubes, rack pack, sterile, flat top caps, graduation |
|||
SGB012-R | SEEN GROUP LIMITED | 25pcs/rack, 500pcs/carton | 50 EUR |
Kimax Centrifuge tubes graduated beaded top white scale 10ml - PK12 |
|||
TUB2708 | Scientific Laboratory Supplies | PK12 | 469.8 EUR |
DB 2.1 Single block for 12 x 15ml conical centrifuge tubes - EACH |
|||
BLO6032 | Scientific Laboratory Supplies | EACH | 183.6 EUR |
50 mL Centrifuge Tube, Self-Standing, Bulk, Sterile, 25/pk, 500/cs |
|||
602072 | NEST Biotechnology | 500 pcs/cs | 73.26 EUR |
Kimax Reuseable Glass Centrifuge tubes plain high strength 15ml - PK6 |
|||
TUB2718 | Scientific Laboratory Supplies | PK6 | 137.7 EUR |
50ml High Speed Centrifuge Tube, Natural cap, Sterile, 25/Bag |
|||
CT790-SN | Bio Basic | 1PK, 25UNIT | 73.57 EUR |
MICRO CENTRIFUGE TUBE, AMBER, 5 ML, 250/1250 |
|||
MCT-500-X | CORNING | 250/pk | 228 EUR |
50ml Conical Centrifuge Tubes, Green Cap, Sterile, 25unit (cardboard rack) |
|||
CT788-GS | Bio Basic | 1PK, 25UNIT | 73.62 EUR |
50ml Conical Centrifuge Tubes, Yellow Cap, Sterile, 25unit (cardboard rack) |
|||
CT788-YS | Bio Basic | 1PK, 25UNIT | 73.62 EUR |
Holder for up to eight 15ml centrifuge tubes, for Bench-Top Shaker |
|||
099ASR815 | Glascol | each | 648 EUR |
Holder for up to eight 50ml centrifuge tubes, for Bench-Top Shaker |
|||
099ASR850 | Glascol | each | 648 EUR |
Corning 15mL PP Centrifuge Tubes Rack Packed with Plug Seal Cap Sterile 50/Rack 500/Case - PK500 |
|||
430052 | Scientific Laboratory Supplies | PK500 | 248.4 EUR |
DB 3.1 Single block centrifuge tube combination 4 x 1.5; 3 x 15 ml and 2 x 50ml - EACH |
|||
BLO6036 | Scientific Laboratory Supplies | EACH | 183.6 EUR |
MICRO CENTRIFUGE TUBE, MAXYCLEAR, 5 ML, 250/1250 |
|||
MCT-500-C | CORNING | 250/pk | 187.2 EUR |
Centrifuge Tube, Conical, 500mL, PP, sterile, with screw-cap, 8 per bag, 5 bags per case, 40/cs |
|||
C1500 | MTC Bio | 40 | 239 EUR |
The uptake and accumulation of [3H]noradrenaline was temperature-sensitive and inhibited by the tricyclic antidepressant desipramine. Membrane vesicles isolated from PC-12 cells represent a useful model for the investigation of the molecular mechanism of the neuronal noradrenaline transport system.